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| Rank | Previous | Company or Trading | Financial Year End | Turnover (£000s) | Pre-Tax | Employee Count |
| 1 | 1 | Dec-07 | £4,079,320 | n/a | n/a | |
| 2 | 2 | Mar-07 | £1,388,700 | £47,200 | 22,000 | |
| 3 | 4 | Dec-07 | £923,226 | £24,272 | 6,565 | |
| 4 | 3 | Dec-07 | £810,467 | £69,251 | 10,899 | |
| 5 | 5 | Dec-07 | £552,000 | n/a | 7,700 | |
| 6 | 52 | Dec-07 | £511,980 | n/a | 9,296 | |
| 7 | 8 | Dec-07 | £478,000 | n/a | 5,849 | |
| 8 | 9 | Dec-07 | £425,342 | £9,884 | 4,032 | |
| 9 | 6 | Dec-07 | £400,000 | n/a | 4,900 | |
| 10 | 13 | Mar-08 | £379,000 | n/a | 4,464 | |
| 11 | 11 | Dec-07 | £334,000 | n/a | n/a | |
| 12 | 10 | Mar-08 | £370,000 | £8,500 | 4,000 | |
| 13 | 12 | Mar-08 | £358,600 | £14,200 | 2,985 | |
| 14 | 14 | Apr-07 | £315,017 | -£20,573 | 7,311 | |
| 15 | 17 | Dec-08 | £193,000 | £14,400 | 1,553 | |
| 16 | 16 | Dec-08 | £184,302 | £7,770 | 1,168 | |
| 17 | 18 | Jan-07 | £183,877 | £9,921 | 1,978 | |
| 18 | 21 | Dec-07 | £181,042 | £4,238 | 2,265 | |
| 19 | 15 | Dec-07 | £180,700 | £4,600 | 2,120 | |
| 20 | 24 | Dec-07 | £180,402 | £21,028 | 1,651 | |
| 21 | 19 | FedEx UK | May-07 | £180,103 | £586 | 1,792 |
| 22 | 20 | Dec-07 | £171,036 | -£9,659 | 3,376 | |
| 23 | 25 | Dec-07 | £132,644 | £4,369 | 539 | |
| 24 | 23 | Nov-06 | £131,963 | £4,184 | 2,369 | |
| 25 | 27 | Dec-07 | £122,320 | £3,567 | 702 | |
| 26 | 30 | Apr-07 | £117,586 | £1,181 | 1,886 | |
| 27 | 36 | Mar-08 | £114,104 | £1,070 | 1,524 | |
| 28 | 28 | Dec-07 | £112,329 | £420 | 1,297 | |
| 29 | 22 | Stobart Group | Feb-08 | £108,840 | £3,521 | 990 |
| 30 | 29 | Mar-07 | £104,426 | £5,879 | 1,087 | |
| 31 | 32 | Dec-07 | £94,911 | £7,243 | 1,751 | |
| 32 | 31 | Apr-08 | £88,088 | £2,525 | 560 | |
| 33 | 33 | Aug-07 | £81,139 | £1,744 | 1,433 | |
| 34 | 35 | Sep-07 | £78,670 | £3,291 | 1,029 | |
| 35 | 37 | Dec-07 | £75,027 | £6,025 | 945 | |
| 36 | 39 | Dec-07 | £68,504 | £4,156 | 556 | |
| 37 | 38 | Dec-07 | £65,102 | £1,936 | 878 | |
| 38 | 45 | Dec-07 | £64,989 | £1,483 | 196 | |
| 39 | 51 | May-08 | £64,599 | £2,263 | 948 | |
| 40 | 49 | Dec-07 | £62,993 | £10,791 | 356 | |
| 41 | NEW | ECM (Vehicle Delivery Services) | Dec-07 | £60,772 | £5,297 | 505 |
| 42 | 42 | Dec-07 | £56,589 | £2,329 | 421 | |
| 43 | 46 | Aug-07 | £54,209 | £2,172 | 491 | |
| 44 | 43 | Dec-06 | £52,686 | £808 | 715 | |
| 45 | 41 | Dec-07 | £52,290 | £1,503 | 485 | |
| 46 | 47 | Aug-07 | £50,374 | £1,920 | 629 | |
| 47 | 48 | Dec-07 | £47,841 | -£4,084 | 273 | |
| 48 | 54 | Cert Octavian Holdings | Jun-07 | £45,305 | -£3,067 | 479 |
| 49 | NEW | Goldstar Transport | Jul-07 | £42,820 | £2,755 | 208 |
| 50 | 88 | Brit European Transport | Dec-07 | £41,587 | £1,684 | 346 |
| 51 | 60 | Sep-07 | £36,208 | £917 | 467 | |
| 52 | 56 | Mar-08 | £35,816 | £539 | 300 | |
| 53 | 57 | Jun-07 | £35,741 | £1,711 | 385 | |
| 54 | 34 | Mar-08 | £34,812 | -£2,570 | 518 | |
| 55 | 59 | Mar-07 | £34,809 | -£12 | 455 | |
| 56 | 61 | Dec-07 | £33,454 | -£218 | 420 | |
| 57 | 55 | Sep-07 | £32,446 | £1,127 | 309 | |
| 58 | 62 | Mar-08 | £31,535 | £1,497 | 354 | |
| 59 | 58 | Dec-06 | £31,031 | -£379 | 235 | |
| 60 | 64 | Apr-07 | £30,752 | £366 | 403 | |
| 61 | 107 | Online | Jan-07 | £30,061 | £732 | 274 |
| 62 | 63 | Dec-07 | £28,470 | £1,148 | 342 | |
| 63 | 70 | Jun-07 | £28,028 | £79 | 480 | |
| 64 | 65 | May-07 | £27,416 | £535 | 451 | |
| 65 | 66 | Ass. Cold Stors & T'port | Dec-07 | £26,981 | -£801 | 377 |
| 66 | 69 | Jun-06 | £26,368 | £2,643 | 275 | |
| 67 | 73 | Mar-07 | £25,761 | -£206 | 341 | |
| 68 | 77 | Sep-07 | £25,340 | £956 | 215 | |
| 69 | 75 | Dec-07 | £25,260 | £3,370 | 123 | |
| 70 | 67 | Oct-07 | £24,920 | £667 | 649 | |
| 71 | 74 | Mar-07 | £24,500 | £69 | 380 | |
| 72 | 83 | Dec-07 | £24,246 | £262 | 468 | |
| 73 | 76 | Dec-07 | £23,541 | £221 | 302 | |
| 74 | 72 | Dec-07 | £23,377 | £133 | 357 | |
| 75 | 78 | Aug-07 | £23,114 | £176 | 299 | |
| 76 | NEW | London City Bond | Sep-07 | £23,062 | £23 | 242 |
| 77 | NEW | Dec-07 | £22,382 | -£113 | 64 | |
| 78 | 84 | Dec-07 | £22,235 | £706 | 501 | |
| 79 | 81 | Mar-08 | £22,031 | £4,633 | 140 | |
| 80 | 82 | Mar-07 | £21,495 | £526 | 278 | |
| 81 | 79 | Dec-07 | £21,373 | £981 | 350 | |
| 82 | NEW | White & Co | Jan-08 | £21,228 | £7 | 464 |
| 83 | NEW | Redhead Freight | Mar-07 | £20,723 | £1,035 | 161 |
| 84 | 85 | Oct-07 | £19,938 | £456 | 268 | |
| 85 | 95 | Sep-07 | £19,936 | £196 | 112 | |
| 86 | 97 | May-08 | £19,935 | £790 | 230 | |
| 87 | 80 | Jun-07 | £19,423 | -£122 | 269 | |
| 88 | 92 | The Bartrum Group | Dec-07 | £19,369 | £1,055 | 215 |
| 89 | 89 | The Potter Group (Holdings) | Apr-07 | £17,749 | £1,290 | 256 |
| 90 | 93 | May-07 | £17,371 | £171 | 134 | |
| 91 | 105 | James Kemball | Dec-07 | £17,242 | £647 | 128 |
| 92 | 100 | Dec-07 | £17,125 | £286 | 221 | |
| 93 | NEW | Arcese UK | Dec-07 | £17,076 | £578 | 72 |
| 94 | 86 | Aug-07 | £17,031 | £235 | 249 | |
| 95 | 94 | May-07 | £16,840 | £1,204 | 254 | |
| 96 | 102 | Deben Transport | Aug-07 | £16,783 | £685 | 157 |
| 97 | 101 | Laser Transport Int | Dec-07 | £16,395 | £133 | 131 |
| 98 | 96 | Dec-07 | £16,251 | -£210 | 152 | |
| 99 | 87 | Fergusons (Blyth) | Sep-07 | £16,069 | £252 | 255 |
| 100 | 98 | Lenham Storage Co | Aug-07 | £16,032 | £139 | 301 |